Standard Depth Slides- The slides are disposable and cannot be reused. Each slide has two chambers. The fixed cover slip creates a constant depth so you get an accurate volume and accurate sperm counts. These slides are non-toxic so samples remain viable and can be counted for up to 45 minutes after loading the chamber. Due to the consistently accurate chamber depth and specially developed coating, no air bubbles will occur when these slides are loaded with a semen sample.

Pre-Stained Morphology Slides - These 1" X 3" slides are used for staining sperm. Each slide contains a dried layer of stain that once in contact with semen, facilitates morphology assessment. These slides do not come with cover slips and cannot be reused. (You can order cover slips below.) There is room for two cover slips on each slide so you get two viewings per slide. Directions for use- Apply a small drop (approximately 2-4 microliters) of semen on the center of the slide. Place the cover slip on top of the slide, exerting slight pressure on the cover slip.

Cell-VU Semen Analysis Chambers - If you are not using a calibrated, Kokopelli Technologies microscope these slides may be helpful. They consist of a specially designed glass slide and cover slip. A counting grid is laser-etched into the surface of the cover slip. These slides are disposable but, since the cover slip is not fixed, they can be washed and reused.
Directions for use- Apply a small drop (approximately 4 microliters) of semen to the center of the slide. (The key to getting accurate results with these slides is to carefully apply a drop of 4 microliters.) Slide the cover slip into place. Count all the sperm within 10 small boxes in the grid. Divide this total number by two to get the concentration of sperm in millions/ml. (Complete directions are included.)

When I tested these slides I did not use a pipette to apply a 4 microliter drop. (A pipetter capable of accurately measuring 4 microliters costs upwards of $150). I found that the sperm counts varied depending on how much sample I put on the slide. Since these slides do not have a fixed cover slip the thickness of the sample can vary. Use a small drop and be careful not to get an air bubble under the slide.- Ken

Hemacytometer for Semen Analysis- We have these made specially for Kokopelli Technologies, LLC. If you are not using a calibrated, Kokopelli Technologies microscope these slides are the perfect solution for getting truly accurate sperm counts. These slides are exactly like a standard hemacytometer except the counting areas are 20 microns deep for sperm counting. An H-shaped moat forms two counting areas, or plateaus. Each plateau features enhanced Neubauer rulings. Contact of the flat, polished cover glass surface with cover glass supports produces an exact volume of fluid over the counting area. These slides are very durable and can be washed and reused countless times. Each slide comes with two reusable cover slips. See instructions.